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MZ503612.1__QYC52355.1__X__00047

Bact-Vir

MZ503612.1__QYC52355.1__X__00047

Identity

Accession:
MZ503612 ↗
Kingdom:
phage

Quality

91.2 mean pLDDT

Cluster

Singleton — not in a non-trivial cluster

3D Structure

Domains

high = three domain segmentations agree, medium = two domain segmentations agree. Numbered high-first then medium, N→C within each tier.

D1 high residues 5-87
PDB
Domain cluster: representative
CATH (19)
Domain IDClass IDClassificationTM-scoreBitsE-valueQ covT cov
2ogqA01 3.30.1120.30 Alpha Beta › 2-Layer Sandwich › Arylsulfatase, C-terminal domain › POLO box domain 0.69 63.0 5.44e-01 100.0% 92.9%
2ysiA01 2.20.70.10 Mainly Beta › Single Sheet › Ubiquitin Ligase Nedd4; Chain: W; › 0.62 26.0 3.70e-01 89.2% 93.9%
1l3aA00 2.30.31.10 Mainly Beta › Roll › Transcriptional Co-activator pc4; Chain A › Transcriptional Coactivator Pc4; Chain A 0.60 41.0 3.34e-01 71.1% 88.0%
1z1bA01 3.30.160.60 Alpha Beta › 2-Layer Sandwich › Double Stranded RNA Binding Domain › Classic Zinc Finger 0.59 32.0 3.68e-01 100.0% 73.7%
3hj4A02 3.30.460.10 Alpha Beta › 2-Layer Sandwich › Beta Polymerase; domain 2 › Beta Polymerase, domain 2 0.58 41.0 3.56e-01 74.7% 58.0%
3lxqA01 3.30.1120.80 Alpha Beta › 2-Layer Sandwich › Arylsulfatase, C-terminal domain › 0.57 42.0 3.97e-01 78.3% 85.9%
1a15A00 2.40.50.40 Mainly Beta › Beta Barrel › OB fold (Dihydrolipoamide Acetyltransferase, E2P) › 0.55 34.0 3.71e-01 84.3% 76.1%
2pm6D01 2.130.10.10 Mainly Beta › 7 Propeller › Methylamine Dehydrogenase; Chain H › YVTN repeat-like/Quinoprotein amine dehydrogenase 0.55 38.0 2.64e-01 72.3% 73.2%
5ic7A00 2.130.10.10 Mainly Beta › 7 Propeller › Methylamine Dehydrogenase; Chain H › YVTN repeat-like/Quinoprotein amine dehydrogenase 0.55 37.0 2.50e-01 71.1% 34.1%
1f1sA03 2.60.220.10 Mainly Beta › Sandwich › Chondroitinase Ac; Chain A, domain 3 › Polysaccharide lyase family 8-like, C-terminal 0.54 29.0 2.96e-01 77.1% 50.0%
1ospO02 3.90.930.1 Alpha Beta › Alpha-Beta Complex › Outer Surface Protein A; domain 3 › 0.53 38.0 3.17e-01 74.7% 86.3%
3bk5A00 2.50.20.10 Mainly Beta › Clam › outer membrane lipoprotein receptor (LolB), chain A › Lipoprotein localisation LolA/LolB/LppX 0.53 37.0 2.77e-01 74.7% 73.2%
4j0xA00 2.130.10.10 Mainly Beta › 7 Propeller › Methylamine Dehydrogenase; Chain H › YVTN repeat-like/Quinoprotein amine dehydrogenase 0.52 42.0 2.77e-01 88.0% 89.6%
1nrkA01 3.30.70.1630 Alpha Beta › 2-Layer Sandwich › Alpha-Beta Plaits › 0.52 43.0 4.10e-01 97.6% 78.4%
4pq0A02 2.30.29.30 Mainly Beta › Roll › PH-domain like › Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) 0.51 37.0 3.65e-01 78.3% 79.6%
3a32A02 3.40.50.800 Alpha Beta › 3-Layer(aba) Sandwich › Rossmann fold › Anticodon-binding domain 0.51 44.0 3.78e-01 100.0% 78.7%
2joiA00 3.30.310.190 Alpha Beta › 2-Layer Sandwich › TATA-Binding Protein › 0.51 44.0 4.20e-01 95.2% 86.5%
5h92B03 3.30.413.10 Alpha Beta › 2-Layer Sandwich › Sulfite Reductase Hemoprotein; domain 1 › Sulfite Reductase Hemoprotein, domain 1 0.51 39.0 3.26e-01 83.1% 66.7%
6m9yA00 3.30.1300.40 Alpha Beta › 2-Layer Sandwich › Pantoate--beta-alanine Ligase; Chain: A,domain 2 › 0.51 26.0 2.96e-01 88.0% 63.2%
ECOD (26)
UIDF-IDClassificationTM-scoreBitsE-valueQ covT cov
3716713 4076.3.1.0 a+b two layers › L9 N-domain-like › GINS/PriA/YqbF domain › GINS/PriA/YqbF domain 0.63 35.0 4.21e-01 80.7% 88.0%
3742752 3257.1.1.1 a+b complex topology › Phosphoinositide phosphatase SAC1 N-terminal domain › Phosphoinositide phosphatase SAC1 N-terminal domain › Phosphoinositide phosphatase SAC1 N-terminal domain › Syja_N 0.62 40.0 2.96e-01 100.0% 26.8%
5061930 220.1.1.0 beta barrels › PH domain-like › PH domain-like › PH domain-like 0.61 38.0 3.65e-01 100.0% 54.7%
3705749 5.1.4.0 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed 0.59 41.0 2.42e-01 72.3% 68.8%
None 0.58 41.0 2.83e-01 72.3% 52.1%
4996952 2492.1.1.2 a+b three layers › Cytidine deaminase-like › Cytidine deaminase-like › Cytidine deaminase-like › JAB 0.58 40.0 3.23e-01 73.5% 78.3%
4022544 5.1.4.1 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed › WD40 0.58 40.0 2.57e-01 72.3% 71.7%
3711234 5.1.4.175 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed › Beta-prop_IFT122_2nd 0.57 40.0 2.78e-01 72.3% 52.1%
3675561 5.1.3.0 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 6-bladed 0.57 40.0 2.69e-01 71.1% 35.6%
4016795 5.1.4.1 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed › WD40 0.56 39.0 2.61e-01 71.1% 47.3%
3896806 5.1.4.341 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed › Beta-prop_IFT140_2nd 0.56 38.0 2.35e-01 71.1% 33.7%
3508084 220.1.1.7 beta barrels › PH domain-like › PH domain-like › PH domain-like › IRS 0.55 36.0 3.64e-01 97.6% 65.9%
3917776 5.1.3.6 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 6-bladed › Kelch_1 0.55 38.0 2.58e-01 72.3% 81.3%
3586726 5.1.4.421 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed › Beta-prop_IFT140_1st, Beta-prop_IFT140_2nd 0.55 38.0 2.63e-01 72.3% 45.8%
4960002 220.1.1.0 beta barrels › PH domain-like › PH domain-like › PH domain-like 0.54 35.0 3.47e-01 100.0% 60.0%
3720799 5.1.5.1 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 8-bladed › WD40 0.54 38.0 2.56e-01 72.3% 80.6%
3804813 5.1.3.65 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 6-bladed › DUF295 0.53 37.0 2.54e-01 71.1% 34.9%
3939425 2492.1.1.8 a+b three layers › Cytidine deaminase-like › Cytidine deaminase-like › Cytidine deaminase-like › UPF0172 0.53 42.0 3.23e-01 86.7% 93.8%
3431397 5.1.3.118 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 6-bladed › FBA_3 0.53 36.0 2.41e-01 71.1% 75.5%
3596879 5.1.4.0 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed 0.53 37.0 2.37e-01 72.3% 54.0%
3465957 5.1.4.504 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed › b-prop_At3g26010-like 0.52 36.0 2.39e-01 73.5% 99.0%
3676177 5.1.5.0 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 8-bladed 0.51 36.0 2.49e-01 72.3% 94.0%
173669 7502.1.1.1 a/b three-layered sandwiches › Anticodon-binding domain of Class II aaRS › Anticodon-binding domain of Class II aaRS › Anticodon-binding domain of Class II aaRS › HGTP_anticodon 0.51 44.0 3.78e-01 100.0% 78.7%
3938865 5.1.4.1 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed › WD40 0.51 37.0 2.53e-01 79.5% 92.3%
3742051 5.1.4.0 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed 0.51 40.0 2.68e-01 85.5% 94.7%
3709573 5.1.4.302 beta duplicates or obligate multimers › beta-propeller-like › beta-propeller › 7-bladed › WD40, Beta-prop_EML 0.50 34.0 2.40e-01 71.1% 60.0%
D2 high residues 97-151
PDB
Domain cluster: representative
CATH (30)
Domain IDClass IDClassificationTM-scoreBitsE-valueQ covT cov
6hj2A00 1.10.565.10 Mainly Alpha › Orthogonal Bundle › Retinoid X Receptor › Retinoid X Receptor 0.63 50.0 3.21e-01 89.1% 27.0%
3ewiB00 3.40.50.1000 Alpha Beta › 3-Layer(aba) Sandwich › Rossmann fold › HAD superfamily/HAD-like 0.62 52.0 3.78e-01 96.4% 94.3%
7c2fB01 3.40.20.10 Alpha Beta › 3-Layer(aba) Sandwich › Severin › Severin 0.61 44.0 3.84e-01 92.7% 50.0%
4kyzA00 3.30.70.600 Alpha Beta › 2-Layer Sandwich › Alpha-Beta Plaits › Ribosomal protein S10 0.61 43.0 3.06e-01 94.5% 25.1%
1wh2A01 3.30.1490.40 Alpha Beta › 2-Layer Sandwich › Dna Ligase; domain 1 › GYF domain 0.60 42.0 4.13e-01 76.4% 73.8%
1qysA00 3.30.1710.10 Alpha Beta › 2-Layer Sandwich › top7, de novo designed protein › top7, de novo designed protein 0.58 43.0 3.61e-01 96.4% 46.7%
7ct1A01 3.10.20.90 Alpha Beta › Roll › Ubiquitin-like (UB roll) › Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 0.58 41.0 3.60e-01 76.4% 94.1%
4gi3C00 3.30.60.30 Alpha Beta › 2-Layer Sandwich › Wheat Germ Agglutinin (Isolectin 2); domain 1 › 0.57 39.0 3.89e-01 70.9% 70.2%
3buxB03 3.30.505.10 Alpha Beta › 2-Layer Sandwich › SHC Adaptor Protein › SH2 domain 0.57 39.0 3.36e-01 76.4% 46.5%
4nlcA01 3.30.420.10 Alpha Beta › 2-Layer Sandwich › Nucleotidyltransferase; domain 5 › Ribonuclease H-like superfamily/Ribonuclease H 0.57 44.0 2.87e-01 85.5% 80.5%
3nyiB01 3.40.50.10170 Alpha Beta › 3-Layer(aba) Sandwich › Rossmann fold › 0.56 45.0 3.39e-01 94.5% 86.6%
2bkfA01 3.10.20.90 Alpha Beta › Roll › Ubiquitin-like (UB roll) › Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 0.56 43.0 3.77e-01 83.6% 84.3%
2kgfA00 1.10.375.10 Mainly Alpha › Orthogonal Bundle › Human Immunodeficiency Virus Type 1 Capsid Protein › Human Immunodeficiency Virus Type 1 Capsid Protein 0.56 40.0 2.95e-01 74.5% 45.7%
2pnqA00 3.60.40.10 Alpha Beta › 4-Layer Sandwich › Phosphatase 2c; domain 1 › PPM-type phosphatase domain 0.56 48.0 2.93e-01 98.2% 58.6%
3weeA01 3.30.420.40 Alpha Beta › 2-Layer Sandwich › Nucleotidyltransferase; domain 5 › ATPase, nucleotide binding domain 0.55 44.0 3.01e-01 85.5% 31.1%
2c60A01 3.10.20.90 Alpha Beta › Roll › Ubiquitin-like (UB roll) › Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 0.55 43.0 3.87e-01 87.3% 87.3%
1sb7A02 3.30.2340.10 Alpha Beta › 2-Layer Sandwich › Pseudouridine synthase › TruD, insertion domain 0.55 38.0 2.97e-01 76.4% 58.8%
3vz9B00 3.30.457.50 Alpha Beta › 2-Layer Sandwich › Copper Amine Oxidase; Chain A, domain 1 › Chromosome segregation protein Spc25 0.54 42.0 3.46e-01 85.5% 54.4%
7kfuC02 1.20.120.920 Mainly Alpha › Up-down Bundle › Four Helix Bundle (Hemerythrin (Met), subunit A) › CRISPR-associated endonuclease Cas1, C-terminal domain 0.53 47.0 3.05e-01 98.2% 30.5%
3bk2A03 3.10.20.580 Alpha Beta › Roll › Ubiquitin-like (UB roll) › 0.53 40.0 3.41e-01 85.5% 69.7%
4dqnA01 3.30.470.10 Alpha Beta › 2-Layer Sandwich › D-amino Acid Aminotransferase; Chain A, domain 1 › Aminotransferase class 4, branched-chain amino acid transferase, N-terminal domain 0.53 43.0 3.16e-01 92.7% 38.0%
2jkgA00 3.30.450.30 Alpha Beta › 2-Layer Sandwich › Beta-Lactamase › Dynein light chain 2a, cytoplasmic 0.53 44.0 3.12e-01 92.7% 90.9%
1wmhA00 3.10.20.90 Alpha Beta › Roll › Ubiquitin-like (UB roll) › Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 0.52 40.0 3.60e-01 87.3% 86.7%
5u3fB01 3.30.470.10 Alpha Beta › 2-Layer Sandwich › D-amino Acid Aminotransferase; Chain A, domain 1 › Aminotransferase class 4, branched-chain amino acid transferase, N-terminal domain 0.52 42.0 3.19e-01 92.7% 42.1%
1o5lA00 2.60.120.10 Mainly Beta › Sandwich › Jelly Rolls › Jelly Rolls 0.52 44.0 3.42e-01 100.0% 93.0%
2mkyA00 3.30.70.1530 Alpha Beta › 2-Layer Sandwich › Alpha-Beta Plaits › Hypothetical protein rpa1041 0.51 29.0 2.90e-01 80.0% 44.8%
6xzqA01 3.40.91.90 Alpha Beta › 3-Layer(aba) Sandwich › Restriction Endonuclease › Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain 0.51 42.0 3.11e-01 96.4% 48.8%
2asfA00 2.30.110.10 Mainly Beta › Roll › Pnp Oxidase; Chain A › Electron Transport, Fmn-binding Protein; Chain A 0.51 39.0 3.04e-01 85.5% 45.6%
2hhzA00 2.30.110.10 Mainly Beta › Roll › Pnp Oxidase; Chain A › Electron Transport, Fmn-binding Protein; Chain A 0.50 39.0 3.04e-01 89.1% 88.4%
1h2iA01 3.30.390.80 Alpha Beta › 2-Layer Sandwich › Enolase-like; domain 1 › DNA repair protein Rad52/59/22 0.50 44.0 3.22e-01 100.0% 37.8%
ECOD (35)
UIDF-IDClassificationTM-scoreBitsE-valueQ covT cov
3272887 221.1.1.0 a+b two layers › beta-Grasp › Ubiquitin-related › Ubiquitin-like 0.71 43.0 3.17e-01 90.9% 25.2%
3470968 109.1.1.0 alpha superhelices › Repetitive alpha hairpins › Glutathione S-transferase (GST)-C › Glutathione S-transferase (GST)-C 0.68 44.0 2.94e-01 89.1% 18.0%
None 0.68 44.0 2.94e-01 89.1% 18.0%
3410496 379.1.1.0 few secondary structure elements › Kazal-type serine protease inhibitors-like › Kazal-type serine protease inhibitors › Kazal-type serine protease inhibitors 0.63 35.0 3.70e-01 87.3% 60.4%
150973 4967.1.1.5 alpha bundles › helical bundle domain in reverse transcriptase-like polymerases › helical bundle domain in reverse transcriptase-like polymerases › helical bundle domain in reverse transcriptase-like polymerases › RdRP_3 0.62 44.0 2.93e-01 74.5% 59.7%
3393570 102.1.1.61 alpha arrays › HhH/H2TH › SAM/DNA-glycosylase › SAM domain-like › NCD1 0.59 39.0 3.34e-01 85.5% 42.2%
3930427 4357.1.1.0 beta barrels › WWE domain › WWE domain › WWE domain 0.59 35.0 3.31e-01 81.8% 49.2%
3726852 2004.1.1.685 a/b three-layered sandwiches › P-loop domains-like › P-loop domains-related › P-loop containing nucleoside triphosphate hydrolases › HET 0.58 42.0 2.73e-01 96.4% 17.2%
153859 379.1.1.0 few secondary structure elements › Kazal-type serine protease inhibitors-like › Kazal-type serine protease inhibitors › Kazal-type serine protease inhibitors 0.57 39.0 3.89e-01 70.9% 70.2%
3988557 2003.1.5.12 a/b three-layered sandwiches › Rossmann-like › Rossmann-related › S-adenosyl-L-methionine-dependent methyltransferases › N6_N4_Mtase 0.57 46.0 2.95e-01 89.1% 18.1%
4809699 3781.1.1.1 a+b two layers › Ribosomal protein L11/L12e N-terminal domain-like › Ribosomal protein L11/L12e N-terminal domain › Ribosomal protein L11/L12e N-terminal domain › Ribosomal_L11_N 0.57 31.0 3.01e-01 92.7% 43.1%
4397568 821.1.1.1 a+b three layers › GIY-YIG endonuclease › GIY-YIG endonuclease › GIY-YIG endonuclease › GIY-YIG 0.57 50.0 4.20e-01 96.4% 80.0%
3798928 59.1.4.2 beta complex topology › triple barrel › triple barrel › TFIID subunits TAF1-TAF7 › DUF3591 0.57 46.0 2.70e-01 89.1% 28.0%
3929494 5001.1.1.41 alpha bundles › Family A G protein-coupled receptor-like › Family A G protein-coupled receptor-like › Family A G protein-coupled receptor-like › 7TM_GPCR_Srw 0.57 48.0 3.01e-01 94.5% 44.2%
3925464 221.1.1.0 a+b two layers › beta-Grasp › Ubiquitin-related › Ubiquitin-like 0.56 43.0 3.78e-01 81.8% 88.7%
4997210 821.1.1.1 a+b three layers › GIY-YIG endonuclease › GIY-YIG endonuclease › GIY-YIG endonuclease › GIY-YIG 0.56 38.0 3.30e-01 70.9% 83.3%
3399251 245.1.1.1 a+b two layers › Ribonuclease PH domain 2-like › Ribonuclease PH domain 2 › Ribonuclease PH domain 2 › RNase_PH_C 0.55 47.0 3.97e-01 98.2% 56.7%
4158495 821.1.1.1 a+b three layers › GIY-YIG endonuclease › GIY-YIG endonuclease › GIY-YIG endonuclease › GIY-YIG 0.55 37.0 3.23e-01 70.9% 84.4%
3942510 821.1.1.1 a+b three layers › GIY-YIG endonuclease › GIY-YIG endonuclease › GIY-YIG endonuclease › GIY-YIG 0.55 37.0 3.17e-01 70.9% 80.0%
3832766 245.1.1.0 a+b two layers › Ribonuclease PH domain 2-like › Ribonuclease PH domain 2 › Ribonuclease PH domain 2 0.54 42.0 3.39e-01 83.6% 99.0%
4025385 2003.1.5.82 a/b three-layered sandwiches › Rossmann-like › Rossmann-related › S-adenosyl-L-methionine-dependent methyltransferases › Methyltransf_31 0.54 36.0 2.57e-01 70.9% 85.6%
4058492 2004.1.1.0 a/b three-layered sandwiches › P-loop domains-like › P-loop domains-related › P-loop containing nucleoside triphosphate hydrolases 0.54 37.0 2.95e-01 72.7% 46.1%
4002132 5001.1.1.1 alpha bundles › Family A G protein-coupled receptor-like › Family A G protein-coupled receptor-like › Family A G protein-coupled receptor-like › 7tm_1 0.53 44.0 2.82e-01 100.0% 72.9%
3593442 221.1.1.0 a+b two layers › beta-Grasp › Ubiquitin-related › Ubiquitin-like 0.53 38.0 3.15e-01 76.4% 54.3%
4259798 4020.1.1.0 a+b two layers › a+b domain in D-aminoacid aminotransferase-like PLP-dependent enzymes › a+b domain in D-aminoacid aminotransferase-like PLP-dependent enzymes › a+b domain in D-aminoacid aminotransferase-like PLP-dependent enzymes 0.53 43.0 3.14e-01 92.7% 36.4%
3618241 382.1.1.0 few secondary structure elements › Snake toxin-like › Snake toxin-like › Snake toxin-like 0.53 38.0 3.33e-01 87.3% 100.0%
365968 4020.1.1.0 a+b two layers › a+b domain in D-aminoacid aminotransferase-like PLP-dependent enzymes › a+b domain in D-aminoacid aminotransferase-like PLP-dependent enzymes › a+b domain in D-aminoacid aminotransferase-like PLP-dependent enzymes 0.52 42.0 3.04e-01 92.7% 34.7%
4580635 2004.1.1.5 a/b three-layered sandwiches › P-loop domains-like › P-loop domains-related › P-loop containing nucleoside triphosphate hydrolases › ABC_tran 0.52 41.0 2.58e-01 85.5% 25.0%
3180654 299.1.1.0 a+b complex topology › YbaK/ProRS associated domain › YbaK/ProRS associated domain › YbaK/ProRS associated domain 0.52 44.0 3.39e-01 98.2% 54.8%
3937515 5001.1.1.1 alpha bundles › Family A G protein-coupled receptor-like › Family A G protein-coupled receptor-like › Family A G protein-coupled receptor-like › 7tm_1 0.52 41.0 2.66e-01 98.2% 83.0%
4950662 1056.1.1.1 a+b two layers › tRNA pseudouridine synthase TruD insertion domain › tRNA pseudouridine synthase TruD insertion domain › tRNA pseudouridine synthase TruD insertion domain › TruD 0.51 36.0 2.53e-01 78.2% 87.1%
5078886 331.3.1.0 a+b two layers › TBP-like › Bet v1-like › Bet v1-like 0.51 43.0 3.46e-01 94.5% 98.2%
3941506 2002.1.1.16 a/b barrels › TIM beta/alpha-barrel › TIM barrels › TIM barrels › Bac_luciferase 0.51 40.0 2.56e-01 94.5% 25.1%
3428945 7581.1.1.25 a/b three-layered sandwiches › Thiolase-like › Thiolase-like › Thiolase-like › FAE1_CUT1_RppA, ACP_syn_III_C 0.51 45.0 3.00e-01 100.0% 36.7%
3579303 4020.1.1.1 a+b two layers › a+b domain in D-aminoacid aminotransferase-like PLP-dependent enzymes › a+b domain in D-aminoacid aminotransferase-like PLP-dependent enzymes › a+b domain in D-aminoacid aminotransferase-like PLP-dependent enzymes › Aminotran_4 0.50 41.0 2.94e-01 92.7% 33.5%